Addgene - pAG192 PIK3CG 3'UTR Plasmid Data

Plasmid 12019: pAG192 PIK3CG 3'UTR

Gene/insert name:
N23 3'UTR
Alt name:
PIK3CG
Insert size:
600
Species:
H. sapiens (human)
GenBank ID:
NM_002649
Entrez Gene:
PIK3CG (PI3CG, PI3K, PI3Kgamma, PIK3)
Vector backbone:
pIS2
(Search Vector Database)
Backbone manufacturer:
pIS2 derived from pRL-SV40 (Promega)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
3700
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
No
5' sequencing primer:
EBV rev primer (GTGGTTTGTCCAAACTCATC) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

PIK3CG 3'UTR in renilla luciferase reporter plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Farh et al (Science. 2005 Dec 16. 310(5755):1817-21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12019" in your Materials and Methods section.