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pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor
(Plasmid #12153)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12153 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-Sin-Puro-Tet-CMV
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RhoA Biosensor
  • Alt name
    6xHis-RBD-CFP-Linker-Citrine-RhoA WT
  • Alt name
    RhoA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, RHO12, RHOH12)
  • Tag / Fusion Protein
    • 6XHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site blunt (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer use insert sequence
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html

Please note: ECFP sequence contains K27R and N165H amino acid changes

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor was a gift from Klaus Hahn (Addgene plasmid # 12153 ; http://n2t.net/addgene:12153 ; RRID:Addgene_12153)
  • For your References section:

    Spatiotemporal dynamics of RhoA activity in migrating cells. Pertz O, Hodgson L, Klemke RL, Hahn KM. Nature. 2006 Apr 20. 440(7087):1069-72. 10.1038/nature04665 PubMed 16547516