pTriEx-RhoA FLARE.sc Biosensor T19N
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12152||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRhoA Biosensor T19N
Alt name6xHis-RBD-CFP-Linker-Citrine-RhoA T19N
SpeciesH. sapiens (human)
Insert Size (bp)2400
MutationRhoA T19N (dominant-negative mutation)*
Entrez GeneRHOA (a.k.a. ARH12, ARHA, RHO12, RHOH12)
/ Fusion Protein
- 6xHis (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer TriExUP (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Protocols for production and use of Hahn lab biosensors and photactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Please reference plasmid 12150 pTRIEX-RhoA Biosensor WT for complete sequence of biosensor
Please note: ECFP sequence contains K27R and N165H amino acid changes
*There is also a previously unreported A88T mutation in this plasmid. It is not known to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTriEx-RhoA FLARE.sc Biosensor T19N was a gift from Klaus Hahn (Addgene plasmid # 12152 ; http://n2t.net/addgene:12152 ; RRID:Addgene_12152)
For your References section:Spatiotemporal dynamics of RhoA activity in migrating cells. Pertz O, Hodgson L, Klemke RL, Hahn KM. Nature. 2006 Apr 20. 440(7087):1069-72. 10.1038/nature04665 PubMed 16547516