Addgene - Pdx-PH2/pGL3 Plasmid Data

Plasmid 12163: Pdx-PH2/pGL3

Gene/insert name:
Pdx1 promoter PH2 domain
Insert size:
138
Species:
M. musculus (mouse)
GenBank ID:
AF334615
Vector backbone:
pGL3
(Search Vector Database)
Vector type:
Luciferase
Backbone size w/o insert (bp):
4818
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
See pGL3 vector List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Domenico Accili
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The forkhead transcription factor Foxo1 links insulin signaling to Pdx1 regulation of pancreatic beta cell growth. Kitamura et al (J Clin Invest. 2002 Dec . 110(12):1839-47. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12163" in your Materials and Methods section.