Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12163||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Growth in Bacteria
Gene/Insert namePdx1 promoter PH2 domain
SpeciesM. musculus (mouse)
Insert Size (bp)138
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer See pGL3 vector (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Pdx-PH2/pGL3 was a gift from Domenico Accili (Addgene plasmid # 12163 ; http://n2t.net/addgene:12163 ; RRID:Addgene_12163)
For your References section:The forkhead transcription factor Foxo1 links insulin signaling to Pdx1 regulation of pancreatic beta cell growth. Kitamura T, Nakae J, Kitamura Y, Kido Y, Biggs WH, Wright CV, White MF, Arden KC, Accili D. J Clin Invest. 2002 Dec . 110(12):1839-47. 10.1172/JCI16857 PubMed 12488434