|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13511||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5800
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Alt nameInsulin-responsive sequence
SpeciesH. sapiens (human)
Insert Size (bp)150
Entrez GeneIGFBP1 (a.k.a. AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer EBV rev primer
- 3′ sequencing primer LucNrev primer (Common Sequencing Primers)
Two oligonucleotides (GCAAAACAAACTTATTTTGAAGCAAAACAAACTTATTTTGAAGCAAAACAAACTTATTTTGAA and TCGATTCAAAATAAGTTTGTTTTGCTTCAAAATAAGTTTGTTTTGCTTCAAAATAAGTTTGTTTTGCGTAC) were annealed together and ligated into the pGL2-Promoter vector (Promega) to create 3×IRS-luciferase. The IRS sequences were derived from human IGFBP-1's IRS.
See "Author's Map" for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3xIRS luciferase was a gift from Kunliang Guan (Addgene plasmid # 13511 ; http://n2t.net/addgene:13511 ; RRID:Addgene_13511)
For your References section:Negative regulation of the forkhead transcription factor FKHR by Akt. Tang ED, Nunez G, Barr FG, Guan KL. J Biol Chem. 1999 Jun 11. 274(24):16741-6. 10.1074/jbc.274.24.16741 PubMed 10358014