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FAS promoter luciferase
(Plasmid #8890)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8890 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fatty Acid Synthetase Promoter
  • Alt name
    FAS promoter
  • Species
    R. norvegicus (rat); unknown - rat or mouse
  • Insert Size (bp)
    245
  • Entrez Gene
    Fasn
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer EBV-rev
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

-220 to +25 fragment of the Fatty Acid Synthetase promoter with regard to the transcription start site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FAS promoter luciferase was a gift from Bruce Spiegelman (Addgene plasmid # 8890 ; http://n2t.net/addgene:8890 ; RRID:Addgene_8890)
  • For your References section:

    Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1. Kim JB, Sarraf P, Wright M, Yao KM, Mueller E, Solanes G, Lowell BB, Spiegelman BM. J Clin Invest. 1998 Jan 1. 101(1):1-9. 10.1172/JCI1411 PubMed 9421459