FAS promoter luciferase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8890||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFatty Acid Synthetase Promoter
Alt nameFAS promoter
SpeciesR. norvegicus (rat); unknown - rat or mouse
Insert Size (bp)245
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer EBV-rev
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
-220 to +25 fragment of the Fatty Acid Synthetase promoter with regard to the transcription start site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FAS promoter luciferase was a gift from Bruce Spiegelman (Addgene plasmid # 8890 ; http://n2t.net/addgene:8890 ; RRID:Addgene_8890)
For your References section:Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1. Kim JB, Sarraf P, Wright M, Yao KM, Mueller E, Solanes G, Lowell BB, Spiegelman BM. J Clin Invest. 1998 Jan 1. 101(1):1-9. 10.1172/JCI1411 PubMed 9421459