FAS promoter luciferase
(Plasmid #8890)

• Depositing Lab: Bruce Spiegelman
• Publication: Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

Backbone

• Vector backbone: n/a
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fatty Acid Synthetase Promoter
• Alt name: FAS promoter
• Species: R. norvegicus (rat); unknown - rat or mouse
• Insert Size (bp): 245
• Entrez Gene: Fasn
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: EBV-rev
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

-220 to +25 fragment of the Fatty Acid Synthetase promoter with regard to the transcription start site.