pSV Sport SREBP-1c dom neg
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8885||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3160
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)1200
MutationY305A amino acid substitution in the active version of SREBP-1c (K312R). Creates a dominant negative version of SREBP-1c.
Entrez GeneSrebf1 (a.k.a. ADD-1, ADD1, SREBP-1, SREBP-1c, Srebp1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
SREBP-1c cloned from a lambda gt11 rat adipocyte cDNA expression library.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSV Sport SREBP-1c dom neg was a gift from Bruce Spiegelman (Addgene plasmid # 8885 ; http://n2t.net/addgene:8885 ; RRID:Addgene_8885)
For your References section:ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Kim JB, Spiegelman BM. Genes Dev. 1996 May 1. 10(9):1096-107. 10.1101/gad.10.9.1096 PubMed 8654925