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Plasmid 12167: p53 S23A targeting vector
  • p53

  • p53 targeting vector

  • M. musculus (mouse)

  • Trp53 (RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)

  • Ser23 mutation (S23A) in exon 2 and puromycin gene flanked by loxP sites in intron 1; vector includes p53 gene locus from intron 1 through exon 6.

  • pBluescript II SK
    (Search Vector Database)

  • Mammalian Expression, Cre/Lox

  • 3000

  • BamHI

  • No

  • XhoI

  • No

  • T3 List of Sequencing Primers

  • T7

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • View map

  • Tyler Jacks

  • UBMTA

Comments: 

Used to generate a mouse with the endogenous p53 serine 23 mutated. Puromycin cassette flanked by loxP sites is in the reverse orientation and contains an EcoR1 site.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Defective apoptosis and B-cell lymphomas in mice with p53 point mutation at Ser 23. MacPherson et al (EMBO J. 2004 Sep 15. 23(18):3689-99. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12167" in your Materials and Methods section.