Addgene - p53 S23A targeting vector Plasmid Data

Plasmid 12167: p53 S23A targeting vector

Gene/insert name:
p53
Alt name:
p53 targeting vector
Species:
M. musculus (mouse)
Entrez Gene:
Trp53 (RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)
Mutation:
Ser23 mutation (S23A) in exon 2 and puromycin gene flanked by loxP sites in intron 1; vector includes p53 gene locus from intron 1 through exon 6.
Vector backbone:
pBluescript II SK
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
3000
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Used to generate a mouse with the endogenous p53 serine 23 mutated. Puromycin cassette flanked by loxP sites is in the reverse orientation and contains an EcoR1 site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Defective apoptosis and B-cell lymphomas in mice with p53 point mutation at Ser 23. MacPherson et al (EMBO J. 2004 Sep 15. 23(18):3689-99. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12167" in your Materials and Methods section.