p53 S23A targeting vector
(Plasmid #12167)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBluescript II SK
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    p53
  • Alt name
    p53 targeting vector
  • Species
    M. musculus (mouse)
  • Mutation
    Ser23 mutation (S23A) in exon 2 and puromycin gene flanked by loxP sites in intron 1; vector includes p53 gene locus from intron 1 through exon 6.
  • Entrez Gene
    Trp53 (a.k.a. RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Used to generate a mouse with the endogenous p53 serine 23 mutated. Puromycin cassette flanked by loxP sites is in the reverse orientation and contains an EcoR1 site.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p53 S23A targeting vector was a gift from Tyler Jacks (Addgene plasmid # 12167)
  • For your References section:

    Defective apoptosis and B-cell lymphomas in mice with p53 point mutation at Ser 23. MacPherson D, Kim J, Kim T, Rhee BK, Van Oostrom CT, DiTullio RA, Venere M, Halazonetis TD, Bronson R, De Vries A, Fleming M, Jacks T. EMBO J. 2004 Sep 15. 23(18):3689-99. 10.1038/sj.emboj.7600363 PubMed 15343266