Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12201||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)900
Entrez GeneCDC42 (a.k.a. CDC42Hs, G25K, TKS)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX-5' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-Cdc42 was a gift from Jonathan Chernoff (Addgene plasmid # 12201 ; http://n2t.net/addgene:12201 ; RRID:Addgene_12201)
For your References section:Identification of a mouse p21Cdc42/Rac activated kinase. Bagrodia S, Taylor SJ, Creasy CL, Chernoff J, Cerione RA. J Biol Chem. 1995 Sep 29. 270(39):22731-7. 10.1074/jbc.270.39.22731 PubMed 7559398