pGEX-2T-Rac1-Q61L
(Plasmid #12979)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Rac1 constitutively active
  • Alt name
    Rac1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    579
  • Mutation
    Q61L Constitutively active
  • GenBank ID
    AF498964
  • Entrez Gene
    RAC1 (a.k.a. MIG5, Rac-1, TC-25, p21-Rac1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    J. Lu
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T-Rac1-Q61L was a gift from Gary Bokoch (Addgene plasmid # 12979)