pGEX-2T-Cdc42-T17N
(Plasmid #12970)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Cdc42 DN
  • Alt name
    Cdc42
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    576
  • Mutation
    T17N Dominant negative
  • GenBank ID
    M57298
  • Entrez Gene
    CDC42 (a.k.a. RP1-224A6.5, CDC42Hs, G25K)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T-Cdc42-T17N was a gift from Gary Bokoch (Addgene plasmid # 12970)