pGEX-Rac1
(Plasmid #12200)

• Depositing Lab: Jonathan Chernoff
• Publication: Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-7.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rac1
• Species: H. sapiens (human)
• Mutation: Starting methionine (M1) removed; F78S compared to GenBank reference sequence NP_008839.2
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX-5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Richard Cerione (Hart, et al: PMID 8276860)
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry