Addgene - pCMV6M-Pak1 Plasmid Data

Plasmid 12209: pCMV6M-Pak1

Gene/insert name:
Pak1
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
PAK1 (PAKalpha)
Fusion protein or tag:
Myc
Terminal:
N terminal on insert
Vector backbone:
pCMV6
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4900
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
TCGTTTAGTGAACCGTCAG List of Sequencing Primers
3' sequencing primer:
GGAACTTCCAAGGCCAGGA
Bacterial resistance(s)
Ampicillin
Growth strain(s)
JM109
Growth temperature (℃):
30
Growth instructions:
30C JM109
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Jonathan Chernoff
Terms and Licenses:
MTA

Comments:

Cloning sites: SalI-myc-BamHI-Pak1-EcoRI.

There is a 2bp insertion in Addgene's quality control sequence compared to depositor's reference sequence. The insertion is not affecting the ORF.

There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells. Sells et al (Curr Biol. 1997 Mar 1. 7(3):202-10. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12209" in your Materials and Methods section.