Plasmid 12262: pLVTH

• Gene/insert name: None
• Vector backbone: pLVTH
  (Search Vector Database)
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox
• Backbone size (bp): 11086
• 5' sequencing primer: See map
• Bacterial resistance(s) Ampicillin
• Growth strain(s) Stbl3
• Growth temperature (℃): 37
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
• High or low copy: High Copy
• Sequence: View sequences (2)
• Map: View map
• Principal Investigator: Didier Trono
• Terms and Licenses: MTA

Comments: 

This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA.

For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid).

Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning.

Packaging plasmid for Trono lab lentiviral vectors is also available at Addgene http://www.addgene.org/rnaitools

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Article: Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz et al (J Virol. 2003 Aug . 77(16):8957-61. PubMed)