|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12262||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 11086
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer See map (Common Sequencing Primers)
This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA.
For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid).
Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning.
Packaging plasmid for Trono lab lentiviral vectors is also available at Addgene http://www.addgene.org/rnaitools
Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLVTH was a gift from Didier Trono (Addgene plasmid # 12262 ; http://n2t.net/addgene:12262 ; RRID:Addgene_12262)
For your References section:Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz M, Trono D. J Virol. 2003 Aug . 77(16):8957-61. 10.1128/JVI.77.16.8957-8951.2003 PubMed 12885912