pLVTH
(Plasmid #12262)

• Purpose: (Empty Backbone)
• Depositing Lab: Didier Trono
• Publication: Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Backbone

• Vector backbone: pLVTH
• Backbone size (bp): 11086
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: See map

Resource Information

• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA.

For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid).

Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning.

Packaging plasmid for Trono lab lentiviral vectors is also available at Addgene http://www.addgene.org/rnaitools

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.