Addgene - pWZL Blast H-Ras G12V E37G Plasmid Data

Plasmid 12277: pWZL Blast H-Ras G12V E37G

Gene/insert name:
H-Ras G12V E37G
Alt name:
HRas
Species:
H. sapiens (human)
Entrez Gene:
HRAS (C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)
Mutation:
Contains G12V activating mutation. The E37G mutation allows binding and activation of Ral-GEFs, but not of Raf or PI3K.
Vector backbone:
pWZL Blast
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Person or lab that originally
cloned the gene/insert:
See Rodriguez-Viciana, P., et al. (1997) Cell 89, pp. 457
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Species- and cell type-specific requirements for cellular transformation. Rangarajan et al (Cancer Cell. 2004 Aug . 6(2):171-83. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12277" in your Materials and Methods section.