pBabe-puro Ras V12
(Plasmid #1768)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    ras V12
  • Alt name
    HrasV12
  • Alt name
    Hras
  • Alt name
    v-Ha-ras
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • Mutation
    G12V, constitutively active
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Scott Lowe
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-puro Ras V12 was a gift from Bob Weinberg (Addgene plasmid # 1768)