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Plasmid 12278: pWZL Blast H-Ras G12V T35S
  • H-Ras G12V T35S

  • HRas

  • H. sapiens (human)

  • HRAS (C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)

  • Contains G12V activating mutation. The T35S mutation allows binding and activation of Raf, but not of PI3K or Ral-GEF.

  • pWZL Blast
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 5100

  • EcoRI

  • No

  • EcoRI

  • No

  • pBABE 5' List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Blasticidin

  • See Rodriguez-Viciana, P., et al. (1997) Cell 89, pp. 457

  • View sequences (1)
  • Bob Weinberg

  • MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Species- and cell type-specific requirements for cellular transformation. Rangarajan et al (Cancer Cell. 2004 Aug . 6(2):171-83. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12278" in your Materials and Methods section.