pWZL Blast H-Ras G12V T35S
(Plasmid #12278)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pWZL Blast
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    H-Ras G12V T35S
  • Alt name
    HRas
  • Species
    H. sapiens (human)
  • Mutation
    Contains G12V activating mutation. The T35S mutation allows binding and activation of Raf, but not of PI3K or Ral-GEF.
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    See Rodriguez-Viciana, P., et al. (1997) Cell 89, pp. 457
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL Blast H-Ras G12V T35S was a gift from Bob Weinberg (Addgene plasmid # 12278)
  • For your References section:

    Species- and cell type-specific requirements for cellular transformation. Rangarajan A, Hong SJ, Gifford A, Weinberg RA. Cancer Cell. 2004 Aug . 6(2):171-83. 10.1016/j.ccr.2004.07.009 PubMed 15324700