pCMX IkB alpha M
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12329||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIkB alpha M
Alt nameI kappa B alpha mutant
SpeciesM. musculus (mouse)
MutationDominant-negative mutant. Many signal transduction pathways resulting in NF-B activation culminate in a serine phosphorylation of IkB on residues 32 and 36. Phosphorylation of the COOH-terminal PEST sequence has been implicated in constitutive turnover of IkB. We combined the NH2- and COOH-terminal phosphorylation mutants into a single cDNA for this plasmid.
Entrez GeneNfkbia (a.k.a. AI462015, Nfkbi)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
IkB alpha M was generated by digestion of plasmids pCMX-IkBS32/36A and pCMX-IkBMutF with Eco NI and Bst EII and ligation of the small S32/36A fragment to the large vector/IkBMutF fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMX IkB alpha M was a gift from Inder Verma (Addgene plasmid # 12329 ; http://n2t.net/addgene:12329 ; RRID:Addgene_12329)
For your References section:Suppression of TNF-alpha-induced apoptosis by NF-kappaB. Van Antwerp DJ, Martin SJ, Kafri T, Green DR, Verma IM. Science. 1996 Nov 1. 274(5288):787-9. 10.1126/science.274.5288.787 PubMed 8864120