PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123360||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8384
- Total vector size (bp) 7289
Modifications to backboneEFS promoter to express puromycin resistance, U6 cassette exchanged to contain LbCpf1 DR sequence
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameEmpty LbCpf1 crRNA cloning cassette
Insert Size (bp)100
- Promoter U6
- Cloning method Gibson Cloning
- 5′ sequencing primer gagggcctatttcccatgat
- 3′ sequencing primer tactattctttcccctgcactgtac (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE was a gift from Sidi Chen (Addgene plasmid # 123360 ; http://n2t.net/addgene:123360 ; RRID:Addgene_123360)
For your References section:In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens. Chow RD, Wang G, Ye L, Codina A, Kim HR, Shen L, Dong MB, Errami Y, Chen S. Nat Methods. 2019 May;16(5):405-408. doi: 10.1038/s41592-019-0371-5. Epub 2019 Apr 8. 10.1038/s41592-019-0371-5 PubMed 30962622