|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123655||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3639
- Total vector size (bp) 5337
Vector typeInsect Expression, Luciferase
Growth in Bacteria
Gene/Insert nameFirefly luciferase
SpeciesPhotinus pyralis (firefly)
Insert Size (bp)1698
- Promoter pIEx
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GATAACCGCGTTGGTTTTA
- 3′ sequencing primer CCAACTCCCATTGTTATTTCTATGCA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypLUC-MCS vector (Agilent Technologies, Santa Clara, CA USA) and pIEx-EGFP (Doug Brackney, The Connecticut Agricultural Experiment Station, New Haven, CT USA)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
The constitutive firefly luciferase expression plasmid pKM19 was generated by amplifying the firefly luciferase gene from pLUC-MCS and cloning it into pIEx-EGFP (a kind gift from Doug Brackney, The Connecticut Agricultural Experiment Station, New Haven, CT USA) after the EGFP sequence was removed by digestion with XhoI and NcoI, using In-Fusion cloning (Takara Biosciences, Mountain View, CA USA).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKM19 was a gift from Kevin Maringer (Addgene plasmid # 123655 ; http://n2t.net/addgene:123655 ; RRID:Addgene_123655)
For your References section:Aedes aegypti (Aag2)-derived clonal mosquito cell lines reveal the impact of pre-existing persistent infection with the insect-specific bunyavirus Phasi Charoen-like virus on arbovirus replication. Fredericks AC, Wallace LE, Russell TA, Davidson AD, Fernandez-Sesma A, Maringer K. bioRxiv April 1, 2019 10.1101/596205