Addgene - 6969 pNOTAGHsp104(K620T) Plasmid Data

Plasmid 1237: 6969 pNOTAGHsp104(K620T)

Gene/insert name:
Hsp104 (K620T)
Alt name:
Hsp104
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
HSP104 (YLL026W)
Mutation:
K620T
Vector backbone:
pNOTAG
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
6526
Cloning site 5':
nde1
Site destroyed during cloning:
No
Cloning site 3':
sac1
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Doug Hattendorf
Sequence: View sequences (1)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers. Shorter et al (Science 2004 Jun 18;304(5678):1793-7. Epub 2004 May 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1237" in your Materials and Methods section.