PurposepiggyBac-based expression of rtTA-M2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124166||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer TCTGCTAACCATGTTCATGC
- 3′ sequencing primer TATAGACAAACGCACACCG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:KA0637_pPBCAG-rtTAM2-IN was a gift from Hans Schöler (Addgene plasmid # 124166 ; http://n2t.net/addgene:124166 ; RRID:Addgene_124166)
For your References section:Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR. Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. 10.1016/j.stem.2018.05.020 PubMed 29910149