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Addgene

pGlomyc-Frat1
(Plasmid #124499)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124499 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGlomyc (based on pCDNA3.1)
  • Backbone manufacturer
    Olivier Destree
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6400
  • Modifications to backbone
    The pGlomyc vector backbone contains 5’ and 3’ globin UTRs and the pCDNA1.1 BamHI-NsiI polylinker in the backbone of pCDNA3.1(+).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Frat1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    860
  • GenBank ID
    NM_008043.3
  • Entrez Gene
    Frat1 (a.k.a. AW060382)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site EcorI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH polyA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Construct was originally cloned in the lab of prof.dr. Anton Berns, Netherlands Cancer Institute.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGlomyc-Frat1 was a gift from Renee van Amerongen (Addgene plasmid # 124499 ; http://n2t.net/addgene:124499 ; RRID:Addgene_124499)
  • For your References section:

    TMEM98 is a negative regulator of FRAT mediated Wnt/ss-catenin signalling. van der Wal T, Lambooij JP, van Amerongen R. PLoS One. 2020 Jan 21;15(1):e0227435. doi: 10.1371/journal.pone.0227435. eCollection 2020. 10.1371/journal.pone.0227435 PubMed 31961879