p259 pCMV-CRE-M (BglII)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12493||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6100
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Alt namemodified Cre
- Cloning method Restriction Enzyme
- 5′ cloning site See map (unknown if destroyed)
- 3′ cloning site See map (unknown if destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The 3' coding sequence
for CRE-M contained in plasmid
p218 was PCR amplified.
The 812 bp amplification
product was digested with BamHI and MluI, gel purified and
ligated together with both the BamHI and MscI fragment from
p218 containing the 5' end of CRE-M and the MscI-MluI
fragment from pBS185 containing a polyA sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p259 pCMV-CRE-M (BglII) was a gift from Jeffrey Green (Addgene plasmid # 12493 ; http://n2t.net/addgene:12493 ; RRID:Addgene_12493)
For your References section:Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Kaczmarczyk SJ, Green JE. Nucleic Acids Res. 2003 Aug 1. 31(15):e86. 10.1093/nar/gng087 PubMed 12888538
Map uploaded by the depositor.