Plasmid 12493: p259 pCMV-CRE-M (BglII)

• Gene/insert name: CRE-M
• Alt name: modified Cre
• Vector backbone: pBS185
  (Search Vector Database)
• Vector type: Mammalian Expression, Cre/Lox
• Backbone size w/o insert: 6100
• Cloning site 5': See map
• Site destroyed during cloning: Unknown
• Cloning site 3': See map
• Site destroyed during cloning: Unknown
• 5' sequencing primer: na
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Map: View map
• Principal Investigator: Jeffrey Green
• Terms and Licenses: MTA

Comments: 

The 3' coding sequence
for CRE-M contained in plasmid
p218 was PCR amplified.
The 812 bp amplification
product was digested with BamHI and MluI, gel purified and
ligated together with both the BamHI and MscI fragment from
p218 containing the 5' end of CRE-M and the MscI-MluI
fragment from pBS185 containing a polyA sequence.

Article: Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Kaczmarczyk et al (Nucleic Acids Res. 2003 Aug 1. 31(15):e86. PubMed)