Addgene - CMV-Brainbow-1.0 L Plasmid Data

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Plasmid 18721: CMV-Brainbow-1.0 L

Gene/insert name:
dTomato ; mCerulean ; EYFP
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Cre/Lox
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Brainbow Plasmids Information (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Joshua Sanes
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

The Author's Map is a representative Brainbow 1.0 construct. The actual fluorescent genes are as indicated in the Gene/insert name. The order of genes is dTomato ; mCerulean ; EYFP (not what is listed on the PDF).

Please see attached PDF for important additional information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Livet et al (Nature. 2007 Nov 1. 450(7166):56-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18721" in your Materials and Methods section.