|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18725||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepUC18+Thy1 promoter
- Backbone size w/o insert (bp) 9188
Vector typeMammalian Expression, Mouse Targeting, Cre/Lox
Growth in Bacteria
Gene/Insert namedTomato ; EYFP ; mCerulean
- Cloning method Restriction Enzyme
- 5′ cloning site NheI / XhoI (destroyed during cloning)
- 3′ cloning site SspI / XhoI (destroyed during cloning)
- 5′ sequencing primer Thy1 F1 primer (TCTGAGTGGCAAAGGACCTTAGG) (Common Sequencing Primers)
The Author's Map is a representative Brainbow 1.0 construct. The actual fluorescent genes are as indicated in the Gene/insert name.
Thy1 promoter. To linearize plasmid and remove vector sequences, EcoRI + PvuI , or alternatively NotI + PvuI are used (Note that PvuI also cuts inside the pUC18 vector).
Please see attached PDF for important additional information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Thy1-Brainbow-1.0 L was a gift from Joshua Sanes (Addgene plasmid # 18725)
For your References section:Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, Lichtman JW. Nature. 2007 Nov 1. 450(7166):56-62. 10.1038/nature06293 PubMed 17972876