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pHAHA
(Plasmid #12517)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12517 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAHA
  • Backbone size (bp) 10231
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • Double HA tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MCS (not destroyed)
  • 3′ cloning site MCS (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

CMV-driven expression vector with an N-terminal double HA tag. See author's sequence for area surrounding the cloning sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAHA was a gift from Tong-Chuan He (Addgene plasmid # 12517)
  • For your References section:

    Increased expression of S100A6 is associated with decreased metastasis and inhibition of cell migration and anchorage independent growth in human osteosarcoma. Luu HH, Zhou L, Haydon RC, Deyrup AT, Montag AG, Huo D, Heck R, Heizmann CW, Peabody TD, Simon MA, He TC. Cancer Lett. 2005 Nov 8. 229(1):135-48. 10.1016/j.canlet.2005.02.015 PubMed 16157226