Addgene - pAd-CCN1 Plasmid Data

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12519: pAd-CCN1
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Plasmid 12519: pAd-CCN1

Gene/insert name:
CCN1
Alt name:
Cyr61
Species:
M. musculus (mouse)
Entrez Gene:
Cyr61 (CCN1, Igfbp10, AI325051)
Vector backbone:
pAdTrace
(Search Vector Database)
Vector type:
Mammalian Expression
Cloning site 5':
KpnI
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Tong-Chuan He
Terms and Licenses:
MTA

Comments:

pAdTrace is an RFP-based shuttle vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: CCN1/Cyr61 is regulated by the canonical Wnt signal and plays an important role in Wnt3A-induced osteoblast differentiation of mesenchymal stem cells. Si et al (Mol Cell Biol. 2006 Apr . 26(8):2955-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12519" in your Materials and Methods section.