pMSCV mSin1.2 HA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12583||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6300
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt namemSin1 transcript variant protein isoform 2
SpeciesH. sapiens (human)
Insert Size (bp)1527
Entrez GeneMAPKAP1 (a.k.a. JC310, MIP1, SIN1, SIN1b, SIN1g)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI/SalI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer MSCV primer (Common Sequencing Primers)
Terms and Licenses
Human mSin1 isoforms were cloned by RT-PCR from mRNA isolated from HEK-293T cells. This plasmid encodes isoform 2. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV mSin1.2 HA was a gift from David Sabatini (Addgene plasmid # 12583 ; http://n2t.net/addgene:12583 ; RRID:Addgene_12583)
For your References section:mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001 PubMed 16919458
Map uploaded by the depositor.