Addgene - BRCA1 (1-304) Plasmid Data

Plasmid 12645: BRCA1 (1-304)

Gene/insert name:
E3-Ring domain, Ub-ligase
Alt name:
BRCA1
Insert size:
912
Species:
H. sapiens (human)
Entrez Gene:
BRCA1 (BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Vector backbone:
pET151D topo
(Search Vector Database)
Backbone manufacturer:
invitrogen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4500
Cloning method:
TOPO Cloning
Cloning site 5':
topo
Site destroyed during cloning:
Unknown
Cloning site 3':
topo
Site destroyed during cloning:
Unknown
5' sequencing primer:
Universal T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Rachel Klevit
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic et al (Mol Cell. 2006 Mar 17. 21(6):873-80. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12645" in your Materials and Methods section.