Addgene - pBABEpuro HA Brca1 Plasmid Data

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Plasmid 14999: pBABEpuro HA Brca1

Gene/insert name:
Brca1
Species:
H. sapiens (human)
Entrez Gene:
BRCA1 (BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert:
5200
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
D. Livingston, DFCI
Sequence: View sequences (1)
Principal Investigator:
Stephen Elledge
Terms and Licenses:
MTA

Comments:

pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Cortez et al (Science. 1999 Nov 5. 286(5442):1162-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14999" in your Materials and Methods section.