Purpose(Empty Backbone) B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||126649||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMouse Targeting
Growth in Bacteria
Copy numberLow Copy
A portion of this plasmid was derived from a plasmid made byDTA negative selection cassette is from Philippe Soriano. p15A backbone is from Sen Wu, Capecchi laboratory.
Terms and Licenses
- Not Available to Industry
Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
AscI and PacI cloning sites near reported Hipp11 locus (Hippenmeyer et al., 2010), a highly recombinogenic locus.
Genomic sequence in pHipp11 plasmids #52544 and #52545 might be from 129/SvJ mouse according to the paper cited above.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pB6-Hipp11-DTA-Capecchi-for was a gift from Mario Capecchi (Addgene plasmid # 126649 ; http://n2t.net/addgene:126649 ; RRID:Addgene_126649)