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Addgene

pET-22b(+)-RNase HIII
(Plasmid #12683)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12683 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-22b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RNase HIII
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    939
  • GenBank ID
    BSU28620
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the His tag is not in frame.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-22b(+)-RNase HIII was a gift from SeWon Suh (Addgene plasmid # 12683 ; http://n2t.net/addgene:12683 ; RRID:Addgene_12683)
  • For your References section:

    Crystallization and preliminary X-ray crystallographic analysis of RNase HIII from Bacillus subtilis. Kwak JE, Lee JY, Han BW, Moon JH, Sohn SH, Park IS, Kim BG, Suh SW. Acta Crystallogr D Biol Crystallogr. 2001 Mar . 57(Pt 3):438-40. 10.1107/S0907444901000440 PubMed 11223525