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library backbone 3'LTR gRNA and barcode
(Plasmid #127397)


Item Catalog # Description Quantity Price (USD)
Plasmid 127397 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
  • Selectable markers
  • Tag / Fusion Protein
    • Puro-mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    library backbone 3'LTR gRNA and barcode was a gift from Xiaowei Zhuang (Addgene plasmid # 127397 ; ; RRID:Addgene_127397)
  • For your References section:

    Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization. Wang C, Lu T, Emanuel G, Babcock HP, Zhuang X. Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 10.1073/pnas.1903808116. Epub 2019 May 13. 10.1073/pnas.1903808116 PubMed 31085639