ZapLck biosensor
(Plasmid #131584)

• Purpose: Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET
• Depositing Lab: Yingxiao Wang
• Publication: Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi: 10.1126/sciadv.aau2001. eCollection 2019 Jun.

Backbone

• Vector backbone: pSIN-EF2-Nanog-Pur
• Backbone manufacturer: Addgene (#16578)
• Backbone size w/o insert (bp): 7575
• Total vector size (bp): 9378
• Modifications to backbone: The insert is between SpeI and EcoRI.
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZapLck biosensor
• Alt name: Lck biosensor
• Species: Synthetic
• Insert Size (bp): 1803

Resource Information

• Supplemental Documents:
  • ZapLck Biosensor_With Features
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry