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pCS2+PhyBΔCreC
(Plasmid #131781)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 131781 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 6961
  • Vector type
    Mammalian Expression, Cre/Lox ; Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PhyBCreC
  • Species
    A. thaliana (mustard weed), Synthetic; Bacteriophage P1
  • Insert Size (bp)
    2847
  • GenBank ID
    816394 2777477
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+PhyBΔCreC was a gift from George Eisenhoffer (Addgene plasmid # 131781 ; http://n2t.net/addgene:131781 ; RRID:Addgene_131781)
  • For your References section:

    CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light. Yen ST, Trimmer KA, Aboul N, Mullen RD, Culver JC, Dickinson ME, Behringer RR, Eisenhoffer GT. bioRxiv 10.1101/823971