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ARR3tk-eGFP/SV40-mCherry
(Plasmid #132360)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 132360 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    7TGC
  • Backbone manufacturer
    Roel Nusse Lab
  • Backbone size w/o insert (bp) 8094
  • Total vector size (bp) 8626
  • Modifications to backbone
    7xtcf binding site of 7TGC replaced with AR binding elements
  • Vector type
    Lentiviral
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AR responsive elements
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    532
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGCCCGAAGGAATAGAAG
  • 3′ sequencing primer AACGGGCCACAACTCCTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ARR3tk-eGFP/SV40-mCherry was a gift from Charles Sawyers (Addgene plasmid # 132360 ; http://n2t.net/addgene:132360 ; RRID:Addgene_132360)
  • For your References section:

    GREB1 amplifies androgen receptor output in human prostate cancer and contributes to antiandrogen resistance. Lee E, Wongvipat J, Choi D, Wang P, Lee YS, Zheng D, Watson PA, Gopalan A, Sawyers CL. Elife. 2019 Jan 15;8. pii: 41913. doi: 10.7554/eLife.41913. 10.7554/eLife.41913 PubMed 30644358