Raf-1 GST RBD 1-149
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13338||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAmersham Pharmacia
- Backbone size w/o insert (bp) 4950
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)450
Entrez GeneRAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
pGEX-2T bacterial expression vector encoding GST followed by N-terminal residues 2-149 of human Raf-1. Contains the Ras-binding domain that binds preferentially to activated Ras-GTP. Used for determination of Ras-GTP formation in mammalian cell lysates.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Raf-1 GST RBD 1-149 was a gift from Channing Der (Addgene plasmid # 13338 ; http://n2t.net/addgene:13338 ; RRID:Addgene_13338)
For your References section:Two distinct Raf domains mediate interaction with Ras. Brtva TR, Drugan JK, Ghosh S, Terrell RS, Campbell-Burk S, Bell RM, Der CJ. J Biol Chem. 1995 Apr 28. 270(17):9809-12. 10.1074/jbc.270.17.9809 PubMed 7730360