mEGFP-HRas S17N
(Plasmid #18665)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    H-Ras
  • Alt name
    HRas
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    S17N mutation in H-Ras
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Tag / Fusion Protein
    • monomeric EGFP (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEGFP-HRas S17N was a gift from Karel Svoboda (Addgene plasmid # 18665)
  • For your References section:

    Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K. Nat Neurosci. 2006 Feb . 9(2):283-91. 10.1038/nn1635 PubMed 16429133