Addgene - mEGFP-HRas S17N Plasmid Data

Plasmid 18665: mEGFP-HRas S17N

Gene/insert name:
H-Ras
Alt name:
HRas
Insert size:
600
Species:
H. sapiens (human)
Entrez Gene:
HRAS (C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)
Fusion protein or tag:
monomeric EGFP
Terminal:
N terminal on insert
Mutation:
S17N mutation in H-Ras
Vector backbone:
pCI
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4000
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Karel Svoboda
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda et al (Nat Neurosci. 2006 Feb . 9(2):283-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18665" in your Materials and Methods section.