Addgene - mRFP-RBD(R59A)-mRFP Plasmid Data

Plasmid 18664: mRFP-RBD(R59A)-mRFP

Gene/insert name:
Ras binding domain of Raf
Alt name:
RBD
Insert size:
250
Fusion protein or tag:
mRFP
Terminal:
N terminal on insert
Fusion protein or tag:
mRFP
Terminal:
C terminal on insert
Mutation:
R59A mutation in RBD
Vector backbone:
pCI
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4000
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
M. Matsuda (Osaka University)
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Karel Svoboda
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda et al (Nat Neurosci. 2006 Feb . 9(2):283-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18664" in your Materials and Methods section.