Addgene - pEGFP-C1-wtVHL Plasmid Data

Gene/insert name:
von Hippel-Lindau
Alt name:
VHL
Insert size:
572
Species:
R. norvegicus (rat)
GenBank ID:
NM_052801
Entrez Gene:
Vhl (Vhlh)
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert:
4693
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
tctcgagctcaagcttcgg List of Sequencing Primers
3' sequencing primer:
tgtggtatggctgattatgatcagtt
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Gentamicin
Person or lab that originally
cloned the gene/insert:
Michael Lerman, NCI-Frederick
Sequence: View sequences (2)
Principal Investigator:
Lucy Anderson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The von Hippel-Lindau tumor suppressor targets to mitochondria. Shiao et al (Cancer Res. 2000 Jun 1. 60(11):2816-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13390" in your Materials and Methods section.