Addgene - pST1374 Plasmid Data

Browse > Keith Joung > pST1374

Plasmid Links

Author's map

Sequence

Related Plasmids

Keith Joung Lab Plasmids

Zinc Finger Consortium Vector Kits

This is commonly
requested with

Print Friendly Email

Plasmid 13426: pST1374

Gene/insert name:		none
Insert size (bp):		Unknown
Fusion proteins or tags:		Flag
Terminal:		N terminal on backbone
Vector backbone:		pST1374 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		5725
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		BamHI
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Keith Joung
Terms and Licenses:		MTA

Comments: Human zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

315 - 602

CMV_immearly_promoter

236 - 852

CMV_fwd_primer

769 - 789

T7_promoter

863 - 881

Orf frame 2

950 - 1576

1589 - 1630

6xHis

1640 - 1657

BGH_rev_primer

1697 - 1680

bGH_PA_terminator

1683 - 1910

f1_origin

1973 - 2279

pBABE_3_primer

2413 - 2393

SV40_enhancer

2615 - 2399

SV40_promoter

2411 - 2680

SV40_origin

2579 - 2656

SV40pro_F_primer

2641 - 2660

EM7_promoter

2774 - 2841

blast

2842 - 3240

SV40_PA_terminator

3401 - 3520

EBV_rev_primer

3489 - 3508

M13_reverse_primer

3582 - 3564

M13_pUC_rev_primer

3603 - 3581

lac_promoter

3646 - 3617

pBR322_origin

4574 - 3955

Orf frame 1

5589 - 4729

Ampicillin

5589 - 4729

AmpR_promoter

5659 - 5631

Unique restriction sites

BglII		12
NdeI		484
SacI		818
NheI		895
NotI		932
XbaI		964
BamHI		980
EcoRI		1101
ApaI		1581
BstBI		1584
AgeI		1634
StuI		2712
FspI		5024

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13426" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.