Addgene - pST1374 Plasmid Data

Plasmid 13426: pST1374

Gene/insert name:
None
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pST1374
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size (bp):
5725
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

Human zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13426" in your Materials and Methods section.