|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13456||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5528
Vector typePlant Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- AcV5 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Plant zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain.
pDW1775 consists of the following components in the given order: Hinc II, SexA I, EcoN I, lambda T0 terminator, T7 primer site, BsiW I, rrnB terminator, modified NOS promoter, Bgl II, Xho I, plant leader, MASS translation signal, SV40 nuclear localization signal, AcV5 epitope tag, Xba I, Xma I, Age I, BamH I, plant optimized Fok I nuclease domain, Sac I, NOS terminator, Apa I, T3 primer site, phage FD gene VIII terminator, Bpu 10 I, modified yeast His3 gene, Pac I, yeast CEN/ARS, Bsu36 I (not unique), modified beta lactamase gene (ampicillin resistance), E. coli Trp A terminator, BstE II, Blp I, PpuM I, Rsr II, PflF I, Bgl I, modified high copy ColEI replicon, and back to Hinc II. This vector can be maintained in E. coli and yeast (Saccharomyces cerevisiae) and is also able to express a zinc finger nuclease in yeast and plants using the modified NOS promoter to drive expression. Additionally, this vector was designed to have minimal expression of the zinc finger nuclease in E. coli to facilitate cloning and reduce toxicity effects.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDW1775 was a gift from Daniel Voytas (Addgene plasmid # 13456 ; http://n2t.net/addgene:13456 ; RRID:Addgene_13456)