Addgene - pCAG-Sox2-IRES-Neo Plasmid Data

Plasmid 13462: pCAG-Sox2-IRES-Neo

Gene/insert name:
Sox2
Insert size:
950
Species:
M. musculus (mouse)
Entrez Gene:
Sox2 (AL606746.1, Sox-2, lcc, ysb)
Mutation:
None
Vector backbone:
pCAG-gw-IRES-Neo
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6300
5' sequencing primer:
CAG-1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Comments:

The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag. If this cDNA is used in a non-tagged construct, a functional protein (missing the first 3 amino acids--MYN) is produced as confirmed by co-immunoprecipitation, reporter assay, and other experiments.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Tokuzawa et al (Mol Cell Biol. 2003 Apr . 23(8):2699-708. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13462" in your Materials and Methods section.