SUPERBLADE5
(Plasmid #134913)

• Purpose: (Empty Backbone) Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)
• Depositing Labs: Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci
• Publication: Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z.

Backbone

• Vector backbone: SUPERBLADE5
• Backbone size (bp): 5194
• Modifications to backbone: Includes stabilizing sgRNA mutations in backbone to increase genome editing efficiency
• Vector type: CRISPR, Synthetic Biology
• Promoter: U6

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • SUPERBLADE5.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Includes stabilizing sgRNA mutations in backbone to increase genome editing efficiency designed using the method outlined in “Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system” (PMID 24360272).