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SUPERBLADE5
(Plasmid #134913)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 134913 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    SUPERBLADE5
  • Backbone size (bp) 5194
  • Modifications to backbone
    Includes stabilizing sgRNA mutations in backbone to increase genome editing efficiency
  • Vector type
    CRISPR, Synthetic Biology
  • Promoter U6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Includes stabilizing sgRNA mutations in backbone to increase genome editing efficiency designed using the method outlined in “Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system” (PMID 24360272).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SUPERBLADE5 was a gift from Philippe Mangeot & Théophile Ohlmann & Emiliano Ricci (Addgene plasmid # 134913 ; http://n2t.net/addgene:134913 ; RRID:Addgene_134913)
  • For your References section:

    Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins. Mangeot PE, Risson V, Fusil F, Marnef A, Laurent E, Blin J, Mournetas V, Massourides E, Sohier TJM, Corbin A, Aube F, Teixeira M, Pinset C, Schaeffer L, Legube G, Cosset FL, Verhoeyen E, Ohlmann T, Ricci EP. Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z. 10.1038/s41467-018-07845-z PubMed 30604748