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Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins.
Mangeot PE, Risson V, Fusil F, Marnef A, Laurent E, Blin J, Mournetas V, Massourides E, Sohier TJM, Corbin A, Aube F, Teixeira M, Pinset C, Schaeffer L, Legube G, Cosset FL, Verhoeyen E, Ohlmann T, Ricci EP
Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
119942BIC-Gag-CAS9encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.
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119971GAG-CRErecExpresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE protein
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120922BICstim-Gag-dCAS9-VPRencodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.
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134912BLADEEmpty backbone for cloning sgRNA sequence to be used in nanoblades system
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134913SUPERBLADE5Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)
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134914BLADE-182sgRNA targeting GFP to be used in nanoblade system
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