BLADE-182
(Plasmid #134914)

Purpose

sgRNA targeting GFP to be used in nanoblade system
Depositing Labs

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci
Publication

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z. ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	134914	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
Unknown
(Search Vector Database)
Total vector size (bp) 3323
Vector type
CRISPR, Synthetic Biology

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
Unknown

Gene/Insert

Gene/Insert name
GFP
gRNA/shRNA sequence
CGAGGAGCTGTTCACCGGGGtgg
Species
Synthetic
Promoter U6

Cloning Information

Cloning method Unknown
5′ sequencing primer Unknown

(Common Sequencing Primers)

Resource Information

Supplemental Documents
- BLADE-182.gb
Terms and Licenses
- UBMTA
Industry Terms
- Not Available to Industry

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

BLADE-182 was a gift from Philippe Mangeot & Théophile Ohlmann & Emiliano Ricci (Addgene plasmid # 134914 ; http://n2t.net/addgene:134914 ; RRID:Addgene_134914)
For your References section:

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins. Mangeot PE, Risson V, Fusil F, Marnef A, Laurent E, Blin J, Mournetas V, Massourides E, Sohier TJM, Corbin A, Aube F, Teixeira M, Pinset C, Schaeffer L, Legube G, Cosset FL, Verhoeyen E, Ohlmann T, Ricci EP. Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z. 10.1038/s41467-018-07845-z PubMed 30604748

BLADE-182 (Plasmid #134914)