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pBABE MDER
(Plasmid #13494)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13494 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    Morgenstern&Land (NAR 18:3587); Available at Addgene
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyoD1-Estrogen Receptor Fusion Protein
  • Alt name
    Mouse Myod1
  • Alt name
    Human Estrogen receptor 1 alpha
  • Alt name
    Myod1
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Mutation
    full length MyoD was fused to aa282-595 of Esr1. Esr1 is inserted between codons 173 and 174 of MyoD.
  • GenBank ID
    NM_010866
  • Entrez Gene
    Myod1 (a.k.a. AI503393, MYF3, MyoD, Myod-1, bHLHc1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Harold Weintraub Lab, Fred Hutchinson Cancer Research Center
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

The Entrez ID for MyoD is NM_010866 ; the Entrez ID for ER is NM_000125.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE MDER was a gift from Stephen Tapscott (Addgene plasmid # 13494 ; http://n2t.net/addgene:13494 ; RRID:Addgene_13494)
  • For your References section:

    Use of a conditional MyoD transcription factor in studies of MyoD trans-activation and muscle determination. Hollenberg SM, Cheng PF, Weintraub H. Proc Natl Acad Sci U S A. 1993 Sep 1. 90(17):8028-32. 10.1073/pnas.90.17.8028 PubMed 8396258