Addgene - pcDNA3.1 FLIPE-600n Plasmid Data

Plasmid 13541: pcDNA3.1 FLIPE-600n

Gene/insert name:
FLIP-E WT
Alt name:
ECFP-ybeJ-Venus
Alt name:
Glutamate/aspartate binding protein
Alt name:
gltI
Insert size:
2400
Species:
E. coli
GenBank ID:
NP_415188
Fusion protein or tag:
ECFP
Terminal:
N terminal on insert
Fusion protein or tag:
Venus
Terminal:
C terminal on insert
Vector backbone:
pcDNA3.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5428
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Venus is from Dr. Atsushi Miyawaki. Please ask Dr. Miyawaki for permission to use Venus in another vector.
Sequence: View sequences (2)
Principal Investigator:
Wolf Frommer
Terms and Licenses:
MTA

Comments:

For more information, see
http://carnegiedpb.stanford.edu/research/fromme...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors. Okumoto et al (Proc Natl Acad Sci U S A. 2005 Jun 14. 102(24):8740-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13541" in your Materials and Methods section.